VECTORS FOR CONTROLLED GENE EXPRESSION

摘要

Disclosed are improved broad host range expression vectors wherein gene expression is tightly regulated by tetracycline. The disclosed tetracycline-responsive vectors provide for the creation of stable cell lines, and a method of controlled gene expression where the transient elimination of gene expression is indicated. Also disclosed are tetracycline regulated adenovirus vectors which may be efficiently propagated in 293 cells, along with other tetracycline regulated viral vectors. Also disclosed are methods of using the tetracycline regulated vectors in the treatment of diseases such as cancer.