发明名称 |
Process for the preparation of human monoclonal antibodies and their use |
摘要 |
The present invention provides a process for the preparation of human monoclonal antibodies from human B cells. According to this process, human antibody-producing B cells are immortalized by infection with an Epstein-Barr virus, or derivative thereof, that expresses Epstein-Barr virus nuclear antigen 2 (EBNA2). EBNA2 function, which is required for immortalization, is subsequently shut off, which results in an increase in antibody production. The invention provides a variety of systems that allow expression of EBNA2 function to achieve immortalization, followed by reduction of EBNA2 expression or function to facilitate antibody production.
|
申请公布号 |
US5798230(A) |
申请公布日期 |
1998.08.25 |
申请号 |
US19960626860 |
申请日期 |
1996.04.03 |
申请人 |
GSF-FORSCHUNGSZENTRUM FUR UMWELT UND GESUNDHEIT GMBH |
发明人 |
BORNKAMM, GEORG WILHELM;EICK, DIRK;KEMPKES, BETTINA;JOCHNER, NICOLA MARIA;STROBL, LOTHAR J. |
分类号 |
C07K16/00;C12P21/08;(IPC1-7):C12P21/04;C12P21/06 |
主分类号 |
C07K16/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|