| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new diaminofluorescein derivative capable of efficiently reacting with nitrogen monoxide under a neutral condition, giving a fluorescent substance excellent in fluorescent strength and useful as a reagent for measuring the nitrogen monoxide, etc. SOLUTION: A new diaminofluorescein derivative represented by formula I (R<1> , R<2> are each an amino group substituted at adjacent positions on the phenyl ring; R<3> , R<4> are each H, an acyl; R<5> to R<8> are each H, a 1-6C alkyl, an aryl, a halogen). The compound of formula I can efficiently react with nitrogen monoxide under a neutral condition, can give a fluorescent substance excellent in fluorescent strength, and is useful as a reagent for measuring the nitrogen monoxide, etc. The compound of formula I is obtained by protecting the amino group of 2,3-dimethyl-5-nitroaniline of formula II (Me is methyl), oxidizing the methyl group, deriving an acid anhydride from the produced dicarboxylic acid, subjecting the derivative to a ring-closing condensation reaction with a resorcinol compound, and subsequently reducing the nitro group of the produced aminonitrofluorescein compound of formula IV.
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