| 摘要 |
PCT No. PCT/GB95/01643 Sec. 371 Date Jan. 6, 1997 Sec. 102(e) Date Jan. 6, 1997 PCT Filed Jul. 12, 1995 PCT Pub. No. WO96/02666 PCT Pub. Date Feb. 1, 1996A method is provided for determining the presence and/or amount of a microorganism and/or its intracellular material present in a sample performed by: (a) exposing the sample to a specific binding agent that has been immobilized upon a solid substrate, the specific binding agent being capable of binding to the microorganism or its intracellular material such that it becomes associated with the solid substrate, (b) exposing the solid substrate to an agent capable of making adenylate kinase associated with the microorganism and/or its intracellular material accessible to solutions applied to the substrate, (c) applying a solution containing adenosine diphosphate (ADP) to the substrate under conditions whereby adenosine triphosphate (ATP) may be produced by any adenylate kinase present, and (d) measuring the amount of adenosine triphosphate (ATP) and relating that to the presence and/or amount of microorganism or intracellular contents.
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