| 摘要 |
<p>The present invention provides a method for distinguishing the presence of dsDNA from ssDNA on the basis of the shape of the waveform generated as a sample of DNA is subjected to cycling denaturing and annealing temperature. Specifically, applicants have discovered that the slope generated by measuring the change in the rate of fluorescence during the annealing portion of the cycle may be used to differentiate between dsDNA and ssDNA. The present method is useful in the identification of dsDNA that has been generated from a specific target organism and has particular application to the detection of food borne pathogenic bacteria.</p> |
