| 摘要 |
The invention provides an isolated DNA segment encoding a domain of HO-endonuclease, said segment being selected from the group consisting of an isolated DNA segment encoding the N-terminus of HO-endonuclease which contains the sequence-specific catalytic nuclease activity of said HO-endonuclease, said DNA fragment comprising at least 755 nt and encoding for at least 251 amino acids, and said endonuclease being characterized by the presence of at least one copy of the dodecapeptide motif LAGLI-DADG and an isolated DNA segment encoding the C-terminus of HO-endonuclease which contains the DNA binding/recognition activity of said HO-endonuclease, said C-terminus comprising 360 nt and encoding for 120 amino acids.
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