发明名称 |
PRODUCTION OF MATURE PROTEINS IN PLANTS |
摘要 |
A method for producing one of the following proteins in transgenic monocot plant cells is disclosed: (i) mature, glycosylated .alpha.1-antitrypsin (AAT) having the same N-terminal amino acid sequence as mature AAT produced in humans and a glycosylation pattern which increases serum halflife substantially over that of mature non-glycosylated AAT; (ii) mature, glycosylated antithrombin III (ATIII) having the same N-terminal amino acid sequence as mature ATIII produced in humans; (iii) mature human serum albumin (HSA) having the same N-terminal amino acid sequence as mature HSA produced in humans and having the folding pattern of native mature HSA as evidenced by its bilirubin-binding characteristics; and (iv) mature, active subtilisin BPN' (BPN') having the same N-terminal amino acid sequence as BPN' produced in Bacillus. Monocot plants cells are transformed with a chimeric gene which includes a DNA coding sequence encoding a fusion protein having an (i) Nterminal moiety corresponding to a rice .alpha.-amylase signal sequence peptide and, (iii) immediately adjacent the C-terminal amino acid of said peptide, a protein moiety corresponding to the mature protein to be produced.
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申请公布号 |
CA2280894(A1) |
申请公布日期 |
1998.08.20 |
申请号 |
CA19982280894 |
申请日期 |
1998.02.13 |
申请人 |
APPLIED PHYTOLOGICS, INC. |
发明人 |
SUTLIFF, THOMAS D.;RODRIGUEZ, RAYMOND L. |
分类号 |
A01H5/00;C07K14/765;C07K14/81;C12N5/10;C12N9/54;C12N15/09;C12N15/57;C12N15/82;C12P21/02;C12R1/91;(IPC1-7):C12N15/82;C12N15/14;C12N15/15 |
主分类号 |
A01H5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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