REACTOR AND METHOD FOR SOLID PHASE PEPTIDE SYNTHESIS

摘要

A solid phase peptide synthesis reactor system (1) and method of operating the reactor are provided. The reactor system (1) includes a basket (17) rotatable about an axis within a housing (7) and a sump/receiver (5) which delivers fluid to, and collects fluid from, the housing (7). The basket (17) has a perforate side (23) wall against which a resin cake (R) for the peptide synthesis is formed. The reactor (7) and sump/receiver (5) form a loop or circuit through which solutions are circulated. The circulation of the solutions prevents the reactor (7) from flooding so that the basket (17) will not be submerged in solution and allows for the use of less liquid. Thus greater amino acid concentrations may be used. The method includes forming a resin cake (R) of uniform depth on the wall (23) of the basket (17), and spraying the solutions against the resin cake while spinning the basket. The solutions will pass through the resin cake (R) and drain to the sump (5) to be circulated or recycled through the system or discharged from the system. Before a subsequent solution is introduced into the reactor system (1), the prior solution is purged from the system to help control exposure time of the peptide to the solutions.