HISTIDINE TAG CHLAMYDIA PNEUMONIAE ANTIGEN POLYPEPTIDE FUSION PROTEIN, ITS PRODUCTION, DNA CODING FOR THE SAME FUSION PROTEIN, RECOMBINANT VECTOR CONTAINING THE DNA, TRANSFORMANT CONTAINING THE RECOMBINANT VECTOR, RECOMBINANT BACULOVIRUS, ITS PRODUCTION AND PRODUCTION OF ANTI-CHLAMYDIA PNEUMONIAE ANTIBODY

摘要

PROBLEM TO BE SOLVED: To obtain the subject new fusion protein excellent in stability, useful for producing an antibody for diagnosing infection with Chlamydia pneumoniae, by bonding a polypeptide of a specific amino acid sequence to a peptide containing a plural number of continuous histidines. SOLUTION: This fusion protein is a new histidine tag Chlamydia pneumoniae polypeptide fusion protein obtained by bonding a polypeptide containing a sequence composed of at least five continuous amino acids in a peptide of the formula directly or through an amino acid sequence to a peptide containing at least 6 continuous histidines, stably maintains antigenicity as a Chlamydia pneumoniae antibody, is readily purified and is useful as an antigen for producing an antibody for a diagnostic for infection with Chlamydia pneumoniae. The fusion protein is obtained by infecting an insect with a baculovirus integrated with a histidine tag Chlamydia pneumoniae antigen fusion protein and expressing the gene in cells of the insect.