| 摘要 |
PROBLEM TO BE SOLVED: To rapidly carry out a microbial test on beer and its semifinished product, etc., with a high sensitivity and good specificity by conducting a polymerase chain reactional(PCR) method using an oligonucleotide prepared so as to specifically hybridize to a 16S ribosome gene of each of the plural bacteria belonging to the genus Lactobacillus as a primer. SOLUTION: An oligonucleotide prepared so as to specifically hybridize to a 16S ribosome gene of each of Lactobacillus brevis, Lactobacillus casei, Lactobacillus coryniformis and Lactobacillus sp. AB No. 74 belonging to bacteria of the genus Lactobacillus g. 5'-CTGATTTCAACAATGAAGC-3') is used as a primer to carry out a PCR method. Each bacterium can definitely be detected and identified within 1-3 days. |
