| 摘要 |
PCT No. PCT/EP98/00081 Sec. 371 Date Sep. 8, 1998 Sec. 102(e) Date Sep. 8, 1998 PCT Filed Jan. 8, 1998 PCT Pub. No. WO98/31790 PCT Pub. Date Jul. 23, 1998This invention relates to a protein having phospholipase activity, which is characterised in that it has the mature sequence of Aspergillus lysophospholipase or a sequence derived therefrom and that it may be cleaved at at least one site, wherein, in the event of cleavage, the restriction fragments are optionally either linked by means of at least one bond cleavable under reducing conditions or at least one of the unlinked restriction fragments has phospholipase activity, and to a process for the production of this protein by fermenting a suitably transformed lysophospholipase-producing host organism in a suitable culture medium and isolating the protein having phospholipase activity from the cell-free culture filtrate, wherein fermentation is performed in the acidic to slightly alkaline range. |
