发明名称 |
PEPTIDES AND PEPTIDE-LOADED ANTIGEN PRESENTING CELLS FOR THE ACTIVATION OF CTL |
摘要 |
Methods for generating antigen-specific cytotoxic T lymphocytes (CTLs) in vitro are presented in conjuntion with methods for using these CTLs for therapy in vivo. The antigen presenting cells (APCs) used herein notably include dentritic cells pretreated with GM-CSF and IL-4. Antigen-specific CTLs were obtained by incubating CD8 preparations in the presence of APCs that bound desired peptides. IL-7 was generally added at day 1 of the incubation; IL-10 was generally added one day later and during restimulation. The invention also comprises methods for using peptide pulsed dentritic cells to identify novel MHC Class I-restricted tumor antigens and subdominant epitopes.
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申请公布号 |
CA2278189(A1) |
申请公布日期 |
1998.08.06 |
申请号 |
CA19982278189 |
申请日期 |
1998.01.30 |
申请人 |
EPIMMUNE INC. |
发明人 |
SOUTHWOOD, SCOTT;SETTE, ALESSANDRO;SIDNEY, JOHN;TSAI, VAN;CELIS, ESTABAN |
分类号 |
A61K35/26;A61K38/00;A61K39/00;A61P31/04;A61P31/10;A61P31/12;A61P31/18;A61P33/06;C12N5/0783;(IPC1-7):C12N5/06;C12N5/08 |
主分类号 |
A61K35/26 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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