发明名称 |
PEPTIDE SYNTHESIS METHOD AND SOLID SUPPORT FOR USE IN THE METHOD |
摘要 |
A method for the solid-phase synthesis of peptides or proteins in high yield and high purity uses a solid support consisting of a functionalized polystyrene-grafted polymer substrate, the grafted polystyrene chains being substantially non-cross-linked and having a chain molecular weight, not including optional non-reactive substituents, of at least 200,000, preferably in the range of 600,0001,200,000 Particularly suitable polymer substrates are substrates of a polyolefin such as polyethylene The method is particularly wellsuited to the compartmentalized synthesis of a multitude of peptides or proteins in a parallel and substantially simultaneous fashion. Preferred embodiments of a solid support for performing the synthesis are prepared from thin polyethylene sheet or film which has been grafted with polystyrene chains in a radical-initiated process in which the polyethylene sheet or film is immersed in a solution of optionally substituted styrene monomer in an alcohol such as methanol, the volume percentage of styrene in the solution preferably being about 30% v/v, and subjected to gamma irradiation
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申请公布号 |
CA1339987(C) |
申请公布日期 |
1998.08.04 |
申请号 |
CA19890610012 |
申请日期 |
1989.08.31 |
申请人 |
FORSKNINGSCENTER RISO |
发明人 |
BERG, ROLF H.;ALMDAL, KRISTOFFER;PEDERSEN, WALTHER BATSBERG;HOLM, ARNE;TAM, JAMES P.;MERRIFIELD, ROBERT BRUCE |
分类号 |
C07K1/04;C07K5/072;C07K7/06;C07K7/08;C07K14/59;C08F255/00;C08F255/02;C08F291/00;G01N33/531;G01N33/545;(IPC1-7):C07K1/04;G01N33/547 |
主分类号 |
C07K1/04 |
代理机构 |
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地址 |
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