METHOD FOR PROTEIN SIALIZATION CONTROL PRODUCED BY CELLULAR CULTURE OF MAMALS

摘要

The invention relates to a new method for the preparation ofglycoproteins from cellular cultures of mammals, the sialic acidcontent in the glycoproteins produced being controlled in a widerange of values by manipulation of the cellular culture medium.The sialic acid content in the glycoproteins is modified bychanges in the cellular culture parameters which results in aspecific productivity of the cells. The osmotic pressure of thecellular culture and the concentration of the transcriptionenhancer are controlled in the preferable options of the inventionduring the production phase of the cellular culture. The inventionalso relates to new products of soluble tumour necrotic factortype 1 - immunoglobulin G1, and to their application for thetreatment of inflammation or indispositions related to the immunesystem.23 claims