发明名称 |
Water channel |
摘要 |
A WCH-1 cDNA probe specific to mRNA expressing a water channel localized in the kidney collecting tubule and complementary to said mRNA, and the sequence has been identified. The same is obtained by a) subjecting a single-chain cDNA prepared from kidney medullary mRNA of a mammal (rat) to PCR using, as degenerate primers, 5'- (T/C)T(T/C/A/G)AA(T/C)CC(T/C/A/G)GC(T/C/A/G)GT (T/C/A/G)AC- 3' (SEQ ID NO:1) and 5'- AA(T/C/A/G)(G/C)(T/A)(T/C/A/G)C(G/T)(T/C/A/G)GC(T/C/A/G) GG(A/G)TT-3' (SEQ ID NO:2), and b) screening a kidney cDNA library of said mammal using a product of said PCR as a probe. WCH-1 protein molecules constituting said water channel can be produced by Escherichia coli producing protein molecules expressed by WCH-1 gene.
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申请公布号 |
US5785986(A) |
申请公布日期 |
1998.07.28 |
申请号 |
US19950448160 |
申请日期 |
1995.05.23 |
申请人 |
MARUMO, FUMIAKI |
发明人 |
FUSHIMI, KIYOHIDE;UCHIDA, SHINICHI;SASAKI, SEI;MARUMO, FUMIAKI |
分类号 |
C12N1/21;A61K9/127;A61K38/00;C07K14/705;C12N15/09;C12N15/12;C12P21/02;C12R1/19;(IPC1-7):A61K9/127;C12N15/00;C07K1/00;C07K1/04 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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