Peptides and compounds that bind to the IL-1 receptor

摘要

Peptides that bind to the interleukin-1 type I receptor (IL-1RtI) can be used to assay the amount of IL-1R, or an IL-1R agonist or antagonist, in a sample and comprise a sequence of amino acids selected from the group consisting of (1) WXXXGZ1W where Z1 is L, I, A, or Q; (2) XXQZ5YZ6XX where Z5 is P or Aze where Aze is azetidine; and Z6 is S, A, V, or L; and (3) Z23NZ24SZ25Z26Z27Z28Z29Z30L where Z23 is D or Y; Z24 is D or S; Z25 is S or W; Z26 is S or Y; Z27 is D or V; Z28 is S or W; Z29 is F or L; and Z30 is D or L; and where each amino acid is indicated by standard one letter abbreviation; and each X can be selected from any one of the 20 genetically coded L-amino acids or the stereoisomeric D-amino acids. Also provided are peptides which bind to the IL-1RtI, which are 11 to 40 amino acids in length, which comprise the core sequence of amino acids:Z31XWZ32Z33Z34Z35Z36QZ37Z38where each letter represents the standard one letter abbreviation for an amino acid or an analog thereof; X is selected from the group of natural or unnatural amino acids; Z37 is a natural or unnatural cyclic amino acid; Z31 is selected from phenylalanine and acetylated phenylalanine; Z32 is a natural or unnatural amino acid; Z33 is selected from proline and pipecolic acid; Z34 is selected from glycine, d-alanine, d-valine, sarcosine and aminoisobutyric acid; Z35 is a natural or unnatural amino acid and Z36 is selected from tyrosine, phosphotyrosine, phenylalanine and tryptophan; and Z38 is selected from tyrosinamide and substituted tyrosinamide.