发明名称 |
Thermophile gene transfer |
摘要 |
We have developed a new gene transfer system for extreme thermophiles of the genus Thermus, including Thermus flavus., using a chromosomal gene, and a thermostable derivative of the kanamycin-resistance gene (kantr2). A plasmid mediated gene-replacement process is used to insert it into the chromosome resulting in the production of Leu- Kmr transformants. This system not only allows stable, single-copy gene insertion into the chromosome of an extreme thermophile, but can be used in the thermo-genetic process described here to generate thermo-stabilized enzymes and proteins for industrial processes. This host-vector environment makes it possible to generate further thermo-stabilizing mutations in the kan gene beyond those levels previously reported.
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申请公布号 |
US5786174(A) |
申请公布日期 |
1998.07.28 |
申请号 |
US19970790309 |
申请日期 |
1997.01.28 |
申请人 |
THERMOGEN, INC. |
发明人 |
WEBER, J. MARK;DEMIRJIAN, DAVID C.;CASADABAN, MALCOLM J.;PAGRATIS, NIKOS C.;VONSTEIN, VERONIKA |
分类号 |
C12N9/04;C12N9/10;C12N15/74;C12N15/90;(IPC1-7):C12P21/02;C12N15/63;C07K14/00;C07H21/04 |
主分类号 |
C12N9/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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