发明名称 |
Recombinant negative strand RNA virus expression systems and vaccines |
摘要 |
Recombinant negative-strand viral RNA templates are described which may be used with purified RNA-directed RNA polymerase complex to express heterologous gene products in appropriate host cells and/or to rescue the heterologous gene in virus particles. Heterologous gene products include peptides or proteins derived from HIV which may be presented by a chimeric influenza virus to generate an immune response that is protective against challenge with HIV. A chimeric virus is described which contains an HIV peptide inserted into an influenza protein and which induced both humoral and cell-mediated immune responses against HIV. The RNA templates are prepared by transcription of appropriate DNA sequences with a DNA-directed RNA polymerase. The resulting RNA templates are of the negative-polarity and contain appropriate terminal sequences which enable the viral RNA-synthesizing apparatus to recognize the template.
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申请公布号 |
US5786199(A) |
申请公布日期 |
1998.07.28 |
申请号 |
US19940323192 |
申请日期 |
1994.10.14 |
申请人 |
THE MOUNT SINAI SCHOOL OF MEDICINE OF THE CITY UNIVERSITY OF NEW YORK |
发明人 |
PALESE, PETER |
分类号 |
A61K39/00;C07K14/11;C07K14/16;C12N7/01;C12N9/12;C12N9/24;C12N15/10;C12N15/11;C12N15/86;(IPC1-7):C12N7/02;C12N15/44 |
主分类号 |
A61K39/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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