Chemiluminescent assay methods and devices for detecting target analytes

摘要

A method which utilize chemiluminescence for analyte detection and the detection of bacteria on surfaces. The method uses a device comprising a sampling portion made of a first adsorbent material, a container, and a second adsorbent material located within the container. The sampling portion collects analytes from a test sample which includes a surface or volume of a liquid. The second adsorbent material holds one or more chemiluminescent components including luciferase enzyme and cofactors in a dry state. In a preferred embodiment, the sampling portion is swabbed over a suspected contaminated surface. A bacteriolytic solution is then added to the adsorbent and releases ATP from any bacteria present. The ATP diffuses into the second adsorbent and mixes with the reagents therein to produce detectable light.