| 摘要 |
The present invention relates to polypeptides produced in yeast; a DNA construct comprising a DNA sequence encoding such polypeptides, vectors carrying such DNA fragments and yeast cells transformed with the vectors, as well as process of producing heterologous proteins in yeast. The DNA construct encoding the polypeptide sequence has the following structure SP-LP-EEGEPK-*polypeptide* wherein SP is a DNA sequence encoding a signal peptide, LP is a DNA sequence encoding a leader peptide, and EEGEPK is an amino acid sequence located as an N-terminal extension of the following *polypeptide* which is a protein or other polypeptide to be produced. The invention is exemplified with expression of N-terminally extended insulin precursor EEGEPK-MI3. |
