Determining spatial structure of proteins from analysis of accessibility of protein side chains

摘要

The spatial structure of a protein (I) of known primary structure is established by: (a) determining the surface accessibility (SA) of protein side groups; (b) determining conformations that are compatible with SA and physically possible, and (c) forming and refining a structural model. SA is examined by mutagenesis and step (c) involves analysis of conformational energies.