Screening combinatorial library complexes in situ by spectroscopy

摘要

A method for detecting at least one substance (ligand) present in a compound library using at least one additional substance (receptor) that binds to the ligand comprises adding to the compound library such a receptor that has substantially higher molecular weight than the ligand to be identified, and performing a spectroscopic measurement technique of the mixture, without isolating the receptor-ligand complex, to detect dipolar resonance phenomena which occur upon a binding of a receptor to a ligand. Preferably the spectroscopic measurement is made by nuclear magnetic resonance (NMR), preferably transfer Nuclear Overhauser Effect (tNOE) spectra are obtained. The spectral analysis of the binding of lectin to an oligosaccharide library is described.