发明名称 |
PROCESS FOR PREPARING PURIFIED DIMER OF BONE-DERIVED FACTOR |
摘要 |
A process for preparing a purified dimer of a bone-derived factor, which comprises subjecting an inclusion body of a bone-derived factor produced by genetic engineering to the following steps a) to e) in sequence: a) the step of treating an inclusion body of a bone-derived factor with an unfolding agent to prepare a solubilized monomer; b) the step of treating the solubilized monomer with a refolding solution to prepare a dimer; c) the step of subjecting the refolded dimer to ultrafiltration and solvent replacement; d) the step of subjecting the dimer solution prepared above to isoelectric precipitation; and e) the step of subjecting the isoelectrically precipitated dimer to reversed phase chromatography.
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申请公布号 |
CA2275464(A1) |
申请公布日期 |
1998.07.09 |
申请号 |
CA19972275464 |
申请日期 |
1997.12.24 |
申请人 |
HOECHST MARION ROUSSEL LTD. |
发明人 |
HONDA, JUN;ANDOU, HIDETOSHI;SUGIMOTO, SHUNJIRO |
分类号 |
C07K14/51;C12N15/09;C12N15/12;(IPC1-7):C12P21/02 |
主分类号 |
C07K14/51 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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