A METHOD OF OOCYTE ENUCLEATION AND PRODUCTION OF RECONSTITUTED EMBRYOS

摘要

The present invention relates to a process for the enucleation of oocytes and the production of cytoplasts and to the use of such cytoplasts and oocytes in a process of nuclear transplantation for the production of nuclear transfer embryos and multiple offspring of genetic similarity. Accordingly, the present invention provides a method for enucleating an oocyte which method includes: providing an oocyte having a polar body, metaphase plate and cytoplasm; subjecting the oocyte to a compound capable of causing attachment of the polar body to the oocyte; and enucleating the oocyte by separating the polar body and a portion of cytoplasm containing the metaphase plate from remaining cytoplasm. In another aspect of the present invention there if provided a method of increasing cytoplasmic volume in an embryonic cell, said method including: providing at least two cytoplast prepared by a method of enucleating an oocyte; providing an embryonic cell; and fusing said cytoplasts with the embryonic cell.