METHOD OF STABILIZING RECOMBINANT HEPATITIS B VIRUS SURFACE PROTEINS FROM YEAST

摘要

Recombinant hepatitis B virus surface proteins produced in yeast are rapidly and efficiently purified from yeast cell extracts in a high pH buffer. The yeast cell extract is heat treated, cooled and the pH is reduced. The surface proteins are then adsorbed onto wide pore silica followed by elution and concentration. This method eliminates the requisite introduction of protease inhibitors, stabilizes the surface protein and improves product yield.