发明名称 |
PURIFIED EUKARYOTIC-INITIATION FACTOR 4E |
摘要 |
The invention provides a method of purifying human eIF-4E protein and amino acid sequence variants thereof having altered binding affinity for capped RNA. Using the described purification, amino acid sequence variants can readily be expressed, purified and tested. Both lowered and enhanced binding affinity variants are useful for modifying protein expression levels in vivo and in vitro.
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申请公布号 |
WO9828325(A1) |
申请公布日期 |
1998.07.02 |
申请号 |
WO1997US23428 |
申请日期 |
1997.12.19 |
申请人 |
EMORY UNIVERSITY |
发明人 |
HAGEDORN, CURT, H.;SPIVAK-KROIZMAN, TALY;XIE, YIPING |
分类号 |
C07K14/47;C12N15/12;(IPC1-7):C07K1/22;C12N15/67;C12N15/87 |
主分类号 |
C07K14/47 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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