| 摘要 |
<p>The invention concerns a method and assay (including a gel-based assay) for specifically identifying inhibitors of muscle protein degradation, comprising using (i) ubiquitin, (ii) a human ubiquitin-carrier E1 protein and (iii) a human ubiquitin-carrier E2 protein, which E2 protein is able to directly ubiquitinylate protein substrates and is expressed in skeletal and/or cardiac muscle (for example, E217k) and means for determining the extent of inhibition of ubiquitin transfer (for example by scintillation proximity assay (SPA)) to a target protein (for example biotinylated histone 2A).</p> |
