| 摘要 |
The present invention is directed to a method of incorporating foreign material into cells that exist in a non-adherent state. In some embodiments, the method employs a microinjection needle together with an adhesive molecule or apparatus capable of immobilizing cells that exist in a non-adherent state onto a substrate or other surface capable of supporting cell attachment to permit introduction of a desired material, such as nucleic acid, into the cell. The non-adherent cells can be genetically altered by the microinjection of nucleic acid sequences (DNA) of sufficient size to permit direct introduction of said nucleic acid into the chromosomal DNA of the cell, without the use of a retrovirus, adenovirus, or other carrier molecule. The invention also thus provides a method of gene therapy insofar as cells modified according to the defined method, particularly undifferentiated cells such as stem cells, may be created to include a gene or fragment thereof sufficient to replace a deficiency or correct an abnormality or deficiency in the cell, and used in a patient by injection of the cells. The corrected nucleic acid then gives rise to genetically corrected differentiated cell species, and hence an approach useful in treating a number of physiological disorders.
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