Method and Apparatus for Immunoassay using Fluorescent Induced Surface Plasma Emission

摘要

A method and apparatus for immunoassays utilizes an improved collection technique of fluorescence induced emissions at the solid phase/liquid phase interface from surface plasmon resonance sensing devices. The method of light collection for the induced emission of surface plasmon resonance based sensing devices involves (a) irradiating the film of the stratified optical system from the substrate side with light that has a wavelength, polarization and angle of incidence appropriate for exciting surface plasmon resonance fluorescence; (b) incubating the sample containing fluorescently labeled molecules with said solid phase substrate film; and (c) employing 360 DEG azimuthal collection of the fluorescence induced emission cone, and monitoring and analyzing the rate or amount by which the detected induced emission intensity changes as binding between the fluorescent or fluorescently labeled molecules and the film progresses.