Plant genes affecting gibberellic acid biosynthesis

摘要

Genes controlling gibberellic biosynthesis are used in genetic engineering to alter plant development. Alterations in the nature or quantity of products of the genes affects plant development. A family of An genes in monocots encodes a cyclase involved in the early steps of gibberellic acid (GA) biosynthesis. Members of the family are identified in wheat, barley, sorghum and maize. Two members of the family, the genes An1 and An2, are identified in maize. The An1 gene is cloned and the function of the gene is characterized. An2 is isolated and identified by homology to An1. Using recombinant genetic technology, GA levels are manipulated. Changes in GA levels alter monocot plant phenotypes, for example, increasing or decreasing height and fertility.