发明名称 |
Method of increasing expression of foreign genes in plant cells |
摘要 |
Disclosed is a method of making recombinant plant cells having reduced variability of expression and increased levels of expression of foreign genes therein. The method comprises (a) providing a plant cell capable of regeneration; (b) transforming the plant cell with a DNA construct comprising an expression cassette, which construct comprises, in the 5' to 3' direction, a transcription initiation region, a structural gene positioned downstream from the transcription initiation region and operatively associated therewith, and a scaffold attachment region positioned either 5' to the transcription initiation region or 3' to the structural gene, the expression cassette subject to the proviso that T-DNA borders are excluded therefrom. DNA constructs and vectors employed in carrying out the foregoing method are also disclosed, along with plant cells and plants produced thereby.
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申请公布号 |
US5773689(A) |
申请公布日期 |
1998.06.30 |
申请号 |
US19950424229 |
申请日期 |
1995.04.19 |
申请人 |
NORTH CAROLINA STATE UNIVERSITY |
发明人 |
THOMPSON, WILLIAM F.;SPIKER, STEVEN L.;ALLEN, GEORGE C.;HALL, JR., GERALD E.;CHILDS, LISA C. |
分类号 |
C07K14/395;C12N15/82;(IPC1-7):C12N5/00;C12N15/00;A01H1/04 |
主分类号 |
C07K14/395 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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