Immunoselection system for cell elution

摘要

The recovery of immunoselected cells is improved by using a specific affinity elution system utilizing members of an immobilized ligand/antiligand pair, with soluble ligand used for elution. In one embodiment, Immobilized heparin adsorbent is coated with biotinylated antithrombin III which is then crosslinked with avidin, thereby greatly increasing the binding affinity of antithrombin III for heparin. The resulting adsorbent effectively captures biotin-labeled target cells and allow facile elution of immunoselected cells with soluble heparin. The cell separation system is capable of both positive and negative selection.