PROCEDURE FOR OBTAINING DNA, RNA PEPTIDES, POLYPEPTIDES, OR PROTEINS BY RECOMBINANT DNA TECHNIQUES

摘要

Procedure for the production of peptides or polypeptides by microbiological means, characterized by the fact that genes which are at least partially composed of stochastic synthetic polynucleotides are produced simultaneously in a common milieu, that the genes thus obtained are introduced into host cells, that the independent clones of the transformed host cells containing these genes are simultaneously cultivated so as to clone the stochastic genes and lead to the production of proteins expressed by each of these stochastic genes, that screening and/or selection is carried out on such clones of transformed host cells in a manner to identify those clones producing peptides or polypeptides having at least one specified property, that the clones so identified are isolated, then grown in a manner so as to produce at least one peptide or polypeptide having the said property.