FLUORESCENT ENZYME SUBSTRATES AND METHOD FOR ASSAYING ENZYMATIC ACTIVITY

摘要

Enzyme substrates each carrying in its molecule a group which is cleaved by the enzymatic reaction and another group which is cleaved by the enzymatic reaction and then forms a coumarin derivative by intramolecular lactonization. A method for assaying enzymatic activity which comprises effecting an enzymatic reaction with the use of the above substrate and then measuring the fluorescence of the coumarin derivative thus formed to thereby determine the enzymatic activity.