发明名称 |
METHOD OF DNA SEQUENCING BY AFFINITY FRACTIONATION AND ARRAY HYBRIDIZATION |
摘要 |
A method for fractionating and sequencing DNA via affinity interaction is provided comprising contacting cleaved DNA to a first array of oligonucleotide molecules to facilitate hybridization between said cleaved DNA and the molecules; extracting the hybridized DNA from the molecules; contacting said extracted hybridized DNA with a second array of oligonucleotide molecules, wherein the oligonucleotide molecules in the second array have specified base sequences that are complementary to said extracted hybridized DNA; and attaching labeled DNA to the second array of oligonucleotide molecules, wherein the labeled re-hybridized DNA have sequences that are complementary to the oligomers. The invention further provides a method for performing multi-step conversions of the chemical structure of compounds comprising supplying an array of polyacrylamide vessels separated by hydrophobic surfaces; immobilizing a plurality of reactants, such as enzymes, in the vessels so that each vessel contains one reactant; contacting the compounds to each of the vessels in a predetermined sequence and for a sufficient time to convert the compounds to a desired state; and isolating the converted compounds from said array. |
申请公布号 |
WO9827229(A1) |
申请公布日期 |
1998.06.25 |
申请号 |
WO1997US23242 |
申请日期 |
1997.12.16 |
申请人 |
THE UNIVERSITY OF CHICAGO |
发明人 |
MIRZABEKOV, ANDREI DARIEVICH;DUBILEY, SVETLANA A.;LYSOV, YURI PETROVICH |
分类号 |
B01L3/00;B01L3/02;C12Q1/68;C40B40/06;C40B60/14;(IPC1-7):C12Q1/68;B05D1/00;C12P19/34;G01N33/553;G01N33/544;C07H21/02;C07H21/04 |
主分类号 |
B01L3/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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