METHOD OF COUPLING LIGANDS TO A SOLID PHASE IN ACIDIC SOLUTION AND ANIONIC SURFACTANT

摘要

The present invention relates to a method for binding antibody or antigen molecules to solid phase. The reactive compound comprising an antibody or antigen molecule and a solide phase are formed by coupling a specific antibody or antigen molecule to a solid phase using a two-step reaction. The coupling is covalent, between the active groups of the solid phase and the antibody, a fragment thereof or the antigen molecule, in the presence of an anionic surfactant, after addding an alcaline/solution to raise the pH to the basic region.