| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new gene coding H chain V region or L chain V region of an antibody capable of recognizing a structure containing sialic acid and fucose, and capable of recognizing a cancer-concerning antigen, useful for production, etc., of an antibody useful for a serodiagnosis of the cancer, etc. SOLUTION: This gene is a new gene coding H chain V region or L chain V region of an antibody capable of recognizing a structure containing sialic acid and fucose, capable of recognizing a cancer concerning antigen and useful for production, etc., of an antibody recognizing a structure containing slalic acid and fucose, an application, etc., of which to a serodiagnosis of the cancer are expected. The gene is obtained by producing hybridoma 2D3 by using an acidic glycolipid extracted from meconium as an immunogen, extracting mRNA from the cells by a conventional method, converting the mRNA to a cDNA, performing a polymerase chain reaction(PCR) by using the obtained cDNA as a template and further using a primer, and amplifying the DNA fragment coding H chain V region or L chain V region of the antibody. |
