发明名称 |
Methods and apparatus for improved luminescence assays using particle concentration, electrochemical generation of chemiluminescence detection |
摘要 |
What is described are methods and apparatus for performing a binding assay for an analyte of interest present in a sample. The methods include the steps of: forming a composition containing said sample, an assay-performance-substance which contains a component linked to a label compound capable of chemiluminescing when triggered, and a plurality of coated magnetic particles capable of specifically binding with the analyte and/or said assay-performance-substance; incubating said composition to form a complex which includes a particle and said labeled component; magnetically collecting said complex at the surface of an electrode; inducing said label to luminesce by contacting it with a trigger, said trigger being formed in-situ by conversion of a precursor molecule upon introduction of electrochemical energy; and measuring the emitted luminescence to measure the presence of the analyte of interest in the sample.
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申请公布号 |
US5770459(A) |
申请公布日期 |
1998.06.23 |
申请号 |
US19940348749 |
申请日期 |
1994.12.01 |
申请人 |
IGEN INTERNATIONAL, INC. |
发明人 |
MASSEY, RICHARD J.;BLACKBURN, GARY F.;WILKINS, ELIZABETH W.;LELAND, JONATHAN K. |
分类号 |
C07D213/22;C07D213/48;C07D213/55;C07D401/12;C07D405/14;C07D473/08;C07F13/00;C07F15/00;C07H21/00;C12Q1/04;C12Q1/68;G01N1/40;G01N21/66;G01N21/69;G01N21/76;G01N33/533;G01N33/543;G01N33/569;G01N33/58;G01N33/76;(IPC1-7):G01N33/53 |
主分类号 |
C07D213/22 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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