发明名称 |
Modification of cryptic splice sites in heterologous genes expressed in fungi |
摘要 |
The present invention relates to methods for obtaining a fungal host cell comprising a nucleic acid sequence encoding a heterologous polypeptide, wherein at least one cryptic splice site is modified in the nucleic acid sequence. The present invention also relates to a nucleic acid sequence(s) with a modified cryptic splice site(s) as well as nucleic acid constructs, vectors, and host cells comprising said nucleic acid sequence(s). The present invention further relates to methods for recombinant production of a polypeptide encoded by said nucleic acid sequence.
|
申请公布号 |
US5770371(A) |
申请公布日期 |
1998.06.23 |
申请号 |
US19960672158 |
申请日期 |
1996.06.27 |
申请人 |
NOVO NORDISK BIOTECH, INC. |
发明人 |
THOMPSON, SHERYL |
分类号 |
C12N1/15;C12N15/67;C12N15/80;(IPC1-7):C12Q1/68;C12P19/34;C07H21/04 |
主分类号 |
C12N1/15 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|