Chromatographic separation column, inner structures thereof, and chromatographic separation

摘要

The invention relates to a chromatographic separation column whose filling material space comprises in the vertical direction at least two zones between which no means that would hinder vertical flow exist and which are divided by means of substantially vertical walls into separate sections. These zones are so dimensioned that the upper edge of the walls in the uppermost zone is below the upper surface of the column filling material in the operating conditions of the column. The invention further relates to inner structures for such a column and to a chromatographic method for separating dissolved constituents by means of said separation column. In this method, a solution is fed alternately with an eluent uniformly across the upper surface of the filling material in the separation column, the solution is allowed to flow through the filling material bed, and the fractions containing the desired products are collected from the liquid flow passed through the filling material.