发明名称 |
JAUNI ŠŪNU ORGANISMI AR SPECIFISKU SAISTĪTO PEPTĪDU PĀRU MIJIEDARBĪBU |
摘要 |
<p>This invention relates to novel modified host cells which express heterologous fused proteins and methods of screening for test samples having peptide-binding activity; wherein the modified host cell comprises: (a) a gene sequence encoding a heterologous fusion protein; said fusion protein comprising a first peptide of a peptide binding pair, or segment of said first peptide, which is joined to either a DNA binding domain or its corresponding transcriptional activation domain of a transcriptional activation protein; (b) a gene sequence encoding a heterologous fusion protein, said fusion protein comprising a second peptide of the peptide binding pair in (a), or a segment thereof, fused to either a DNA binding domain or its corresponding transcriptional activation domain, whichever one is not employed in (a); (c) a reporter gene operatively associated with the transcriptional activation protein, or a portion thereof; (d) optionally, a deletion or mutation in the chromosomal DNA of the host cell for the transcriptional activation protein if present in the selected host cell.</p> |
申请公布号 |
LV11906(B) |
申请公布日期 |
1998.06.20 |
申请号 |
LV19970000004 |
申请日期 |
1997.02.14 |
申请人 |
AMERICAN CYANAMID COMPANY |
发明人 |
YOUNG,KATHLEEN, H.;OZENBERGER,BRADLEY, A. |
分类号 |
C07K14/61;C07K14/72;C12N1/19;C12Q1/68;(IPC1-7):C12N15/00;C12N15/18;C12N15/62 |
主分类号 |
C07K14/61 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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