METHOD OF QUICK DETERMINING STATE OF ORGAN BASED ON DETECTION OF ONE OR MORE GLUTATHIONE S-TRANSFERASE ISOENZYMES

摘要

PCT No. PCT/IE96/00019 Sec. 371 Date Oct. 3, 1997 Sec. 102(e) Date Oct. 3, 1997 PCT Filed Apr. 2, 1996 PCT Pub. No. WO96/31779 PCT Pub. Date Oct. 10, 1996The present invention is drawn to a method for the rapid assessment of organ status, including organ damage following immunological or toxicological insult, in a subject based on the detection of one or more isoenzymes of glutathione S-transferase (GST) in diverse biological fluids comprising contacting a particle-labelled anti-GST antibody specific for said isoenzyme with a sample of a biological fluid suspected of containing said isoenzyme, said antibody having a sensitivity sufficient to detect at least a picomolar amount of said isoenzyme, and capturing the particle-labelled antibody-isoenzyme complex on an immobolised capture antibody to generate a visually detectable signal.