发明名称 |
Replication of hepatitis C virus genome and identification of virus having high infectivity |
摘要 |
A method for replication of hepatitis C virus genome comprising infecting animal cells that have been infected with mouse retrovirus, with hepatitis C virus, and then culturing those cells. The present invention further provides a method for distinguishing a hepatitis C virus with high infectivity and a hepatitis C virus with less infectivity, comprising centrifugating a sample comprising a virus to be tested in a liquid medium to determine the density of the virus, and assigning the virus to a hepatitis C virus with high infectivity it the density thereof is up to about 1.06 g/ml, and assigning the virus to a hepatitis C virus with less infectivity if the density thereof is at least 1.13 g/ml; a method for distinguishing a hepatitis C virus with high infectivity and a hepatitis C virus with less infectivity, comprising bringing a virus to be tested into contact with an anti-human immunoglobulin, and assigning the virus to hepatitis C virus with high infectivity if the virus does not form an immunocomplex and assigning the virus to a hepatitis C virus with less infectivity if it does form an immunocomplex; and a method for distinguishing a hepatitis C virus with high infectivity and a hepatitis C virus with less infectivity, comprising inoculating a virus to be tested to cells infected with murine retrovirus, culturing the cells, and assigning the virus to a hepatitis C virus with high infectivity if the virus replicates in the cells, or assigning the virus to a hepatitis C virus with less infectivity if the virus does not replicate in the cells.
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申请公布号 |
US5766919(A) |
申请公布日期 |
1998.06.16 |
申请号 |
US19960654128 |
申请日期 |
1996.05.28 |
申请人 |
YOSHIKURA, HIROSHI;SHIMIZU, YOHKO |
发明人 |
YOSHIKURA, HIROSHI;SHIMIZU, YOHKO;IWAMOTO, AIKICHI;HIJIKATA, MINAKO |
分类号 |
C12N7/00;C12Q1/04;G01N33/50;G01N33/576;(IPC1-7):C12N7/00;C12N7/01 |
主分类号 |
C12N7/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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