发明名称 |
Cysteine-pegylated proteins |
摘要 |
Methods and compositions are provided for the production of PEGylated proteins having polyethylene glycol covalently bound to a cysteine residue present in either the naturally-occurring protein or introduced by site-specific mutation. Where the cysteine residue is introduced by mutation, the site for mutation is selected on the basis of the presence of an N-linked glycosylation site or the position of the residue which is normally solvent-accessible in the naturally-occurring protein. The modified proteins produced by the method of the invention are referred to as cysteine-PEGylated proteins. Proteins PEGylated according to the invention have increased half-lives following administration to a subject and decreased immunogenicity and antigenicity, while retaining substantially the same level of biological activity as that of the naturally-occurring, unmodified protein. Modification of proteins according to methods of the invention thus provide improved pharmaceutical compositions.
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申请公布号 |
US5766897(A) |
申请公布日期 |
1998.06.16 |
申请号 |
US19950427100 |
申请日期 |
1995.04.21 |
申请人 |
INCYTE PHARMACEUTICALS, INC. |
发明人 |
BRAXTON, SCOTT M. |
分类号 |
A61K38/00;C07K1/107;C07K14/81;(IPC1-7):C12N15/00;C12N9/96;C12N9/48;C12N9/50 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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