发明名称 |
Kits for suppressing inhibition of enzyme-mediated reactions by ionic detergents using high concentrations of non-ionic detergents |
摘要 |
Methods and kits for the use of a non-ionic detergent to suppress enzyme inhibition in a reaction solution due to the presence of inhibiting ionic detergent. Prior to reaction, the reaction mixture is given an effective amount of a non-ionic detergent, and agitated. The enzyme is then added, and the enzymatic reaction is the allowed to proceed. Also disclosed are preferred embodiments of the present invention, and kits for nucleic acid amplification of a biological sample in the presence of an ionic detergent.
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申请公布号 |
US5766890(A) |
申请公布日期 |
1998.06.16 |
申请号 |
US19970779341 |
申请日期 |
1997.01.06 |
申请人 |
GEN-PROBE INCORPORATED |
发明人 |
KACIAN, DANIEL LOUIS;MCALLISTER, DIANE LISA |
分类号 |
C12N15/09;C12N9/12;C12Q1/25;C12Q1/68;(IPC1-7):C12P19/34 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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