| 摘要 |
PCT No. PCT/US94/00424 Sec. 371 Date Aug. 22, 1995 Sec. 102(e) Date Aug. 22, 1995 PCT Filed Jan. 12, 1994 PCT Pub. No. WO94/16332 PCT Pub. Date Jul. 21, 1994A method for screening or detection of a non-enzyme catalytic polypeptide or protein for the conversion of a substrate S to a product P is provided, in which a preparation containing the potential catalyst is contacted with the substrate S immobilized to a support and the immobilized product P obtained is detected, preferably by immunoassay. The method is preferably used for the screening of hybridoma supernatants for catalytic monoclonal antibodies that catalyze acyl transfer reactions, e.g., hydrolysis or aminolysis, condensation reactions and resolution of enantiomers.
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