发明名称 |
Method of removing N-terminal amino acid residues from eucaryotic polypeptide analogs and polypeptides produced thereby |
摘要 |
A method of sequentially removing one or more N-terminal amino acid residues from an analog of a eucaryotic polypeptide synthesized in a foreign host, comprises contacting the eucaryotic polypeptide analog with aminopeptidase under suitable conditions permitting sequential removal of N-terminal amino acid residues, where the polypeptide analog contains an amino acid residue or sequence of residues which blocks the action of an aminopeptidase located at a position other than the N-terminal end of the polypeptide analog. A specific embodiment of the invention concerns removing both an N-terminal methionine residue and its adjacent Leucine residue from a growth hormone analog produced in bacteria by contacting the growth hormone analog with Aeromonas aminopeptidase under suitable conditions permitting removal of the N-terminal methionine and Leucine residues. The invention also concerns eucaryotic polypeptide analogs produced in accordance with the methods of the invention.
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申请公布号 |
US5763215(A) |
申请公布日期 |
1998.06.09 |
申请号 |
US19950400544 |
申请日期 |
1995.03.08 |
申请人 |
BIO-TECHNOLOGY GENERAL CORPORATION |
发明人 |
BLUMBERG, SHMARYAHU;BEN-MEIR, DANIELLA |
分类号 |
C07K14/61;(IPC1-7):C12P21/02;C12N9/48;C12N15/01;C12N15/09 |
主分类号 |
C07K14/61 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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