发明名称 |
Inhibiting degradation of a degron-bearing protein |
摘要 |
Disclosed herein is a heat-inducible N-degron module. A heat-inducible N-degron module is a protein or peptide bearing a destabilizing N-terminal amino acid residue which becomes a substrate of the N-end rule pathway only at a temperature high enough to result in at least partial unfolding of the protein. At this elevated (nonpermissive) temperature, the heat-inducible N-degron module (and any protein or peptide attached at its C-terminus) is rapidly degraded in a cell in which the N-end rule pathway is operative. Also disclosed are DNA and protein fusion constructs, methods for screening for additional heat-inducible N-degron modules and methods for using the disclosed heat-inducible N-degron modules. Also disclosed are methods for inhibiting the degradation of a protein bearing an N-degron in which a low molecular mass, cell penetrating ligand is prebound to the protein.
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申请公布号 |
US5763212(A) |
申请公布日期 |
1998.06.09 |
申请号 |
US19950417791 |
申请日期 |
1995.04.06 |
申请人 |
CALIFORNIA INSTITUTE OF TECHNOLOGY |
发明人 |
VARSHAVSKY, ALEXANDER;JOHNSTON, JENNIFER |
分类号 |
C12N15/09;C07K14/00;C07K14/395;C07K14/47;C07K19/00;C12N1/21;C12N9/02;C12N9/06;C12N9/12;C12N15/11;C12N15/62;C12P21/02;C12P21/04;C12Q1/68;C12R1/865;(IPC1-7):C12P21/02;C12N5/10 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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