| 摘要 |
PCT No. PCT/EP94/03547 Sec. 371 Date Apr. 29, 1996 Sec. 102(e) Date Apr. 29, 1996 PCT Filed Oct. 28, 1994 PCT Pub. No. WO95/11970 PCT Pub. Date May 4, 1995The invention relates to a process for the production of a primer for consecutive DNA sequencing, in which at least two shortmers are hybridised adjacent to one another using a template and are then ligated with one another to form the primer, the process being characterised in that (a) a template in the form of a single strand of nucleotides is used in which the number of nucleotides is at least 6 and is not greater than approximately twice the total number of nucleotides of all the shortmers used, and (b) the primer formed is then separated from the template and from unreacted shortmers and recovered. The invention relates also to banks (libraries) of templates for the mentioned process.
|
