LIGAND DETECTION SYSTEM AND METHODS OF USE THEREOF

摘要

The present invention relates to novel ligand detection systems and methods of using the systems to identify ligands capable of specifically binding orphan protein domains. The invention also relates to peptide ligands capable of specifically binding an orphan domain of interest such as the PDZ domain of neuronal nitric oxide synthase (nNOS). Further provided are methods of detecting the peptide ligands and those orphan protein domains capable of specifically binding the peptide ligands. The present invention is useful for a variety of applications including detecting peptide ligands with therapeutic capacity to treat human diseases.